2 heaviest offspring’ of Rattus norvegicus taken, and their brain tissues are taken as samples. On 20th day of pregnancy, the mothers were dissected using sectio caesarea. Subjects were super ovulated, and 65 dB intensity of music was played for an hour at 8-9 pm on the subjects on 10th day of pregnancy. Subjects were female pregnant Rattus norvegicus, grouped into 3 random groups: 1 control group and 3 treatments groups with 6 samples each. Laboratory experimental research, posttest-only control group design. During pregnancy, Beethoven music is proven to decrease the apoptosis of neuronal. Exposure to classical music such as Beethoven can increase the wave of brain activity. Transfer of these germinants to gelled media withoutPGR's led to the development of normal plantlets.The efforts that can be done to improve brain cells in the prenatal period is the provision of nutrients and good stimulation. The media were supplemented with0.1-2.0 mg/l BAP and 0.1-2.0 mg/l kinetin in combination with0.01 mg/l ABA each for supporting growth and development.The cultures was incubated at 26+1oC under a 12-hphotoperiod with lighting providing an intensity 20 μmolesphotons/m2/second for 11 weeks with replication 10 times.The results showed that the highest of somatic embryoproliferation was achieved in a culture medium with BAP at0.5 mg/l + 0.01 mg/l ABA with an expression rate of 94%, thebest maturation at 1.0 mg/l kinetin + 0.01 mg/l ABA with anexpression rate of 93.5% and the most germination at 2.0mg/l kinetin + 0.01 mg/l ABA with an expression rate of100%. pH of media was adjusted at5.6 before sterilized. Cotyledonstageof somatic embryo derived from shoot tip culturescultured on Modified Murashige-Skoog (MMS) with halfstrengthmacro-salts and added with 30 g/l sucrose, 2 g/lgelrite, 1 g/l activated charcoal.
Kindsand concentration of plant growth regulators (PGR's)influence to growth and development of somatic embryos.The research was conducted to determine the optimal concentrationof BAP and kinetin for proliferation, maturationand germination of sago palm somatic embryos.
Somatic embryos induction in sago palm(Metroxylon sagu Rottb.) have succesfully developed.
Effect of Kinetin and BAP to Growth and Development ofSomatic Embryos of Sago Palm (Metroxylon sagu Rottb.).Imron Riyadi.
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